College of Agriculture 
        and Life Sciences
 Department of Nutritional Sciences

Education:
Doctorate: 1962, University of Wisconsin, Biochemistry - Animal Science
Master of Science: 1959, Iowa State University - Animal Science
Bachelor Degree: 1957, Iowa State University, B.S.- Animal Science

Principal Academic Activities:

NSC665 Biochemistry
NSC (NUSC) 696B Seminar
Biochemistry 498

Research Activities:
Research has focused on the role of protein degradation in growth and muscle diseases, with emphasis on the calpain system, and on properties of cytoskeletal proteins involved in membrane/cytoskeleton interaction, with emphasis on Z-disks in striated muscle.

Representative Publications:

Thompson, V.F., and Goll, D.E. (2000). Purification of µ-calpain, m-calpain and calpastatin from animal tissues. In “Methods in Molecular Biology”, Calpain Methods and Protocols (Elce, J.S., ed.), Humana Press, Inc., Totowa, New Jersey pp. 3-16.

Thompson, V.F., Lawson, K., and Goll, D.E. (2000) Effect of µ-calpain on m-calpain. Biochem. Biophys. Res. Comm. 267, 495-499. 

 Bialkowska, K., Kulkani,S., Du, X., Goll,D.E., Saido,T.C., and Fox, J.E.B. (2000) Evidence that $3 integrin-induced Rac activation involves the calpain-dependent formation of integrin clusters that are distinct from focal complexes and focal adhesions that form as Rac and RhoA become active. J. Biol. Chem. 151, 685-695.

Thompson, V.F., Saldańa, S.,Cong, J., and Goll, D.E. (2000) A BODIPY fluorescent microplate assay for measuring activity of calpains and other proteases. Anal. Biochem. 279, 170-178.

Delgado, E.F., Geesink,G.H., Marchello, J.A., Goll, D.E., and Koohmaraie,M. (2001) The calpain system in three muscles of normal and callipyge sheep. J. Anim. Sci. 79, 398-412. 

Delgado, E.F., Geesink, G.H., Marchello, J.A., Goll,D.E., and Koohmaraie, M. (2001) Properties of myofibril-bound calpain activity in longissimus muscle of callipyge and normal sheep. J. Anim. Sci. 79 (in press). 

Cottin, P., Thompson, V.F., Sathe, S.K., Szpacenko, A., and Goll, D.E. (2001) Autolysis of µ- and m-calpain from bovine skeletal muscle. Biol. Chem. (in press).

Interdisciplinary or team research activities. 

           1.      Molecular Mechanisms Regulating Skeletal Muscle Growth and Differentiation.

     This is a USDA Regional Research project that involves cooperative research among 14 experiment stations nationwide and two USDA/ARS research stations (Clay Center, NE and Beltsville, MO); hence, it is a national committee and not limited to one region as is customary. Reagents and procedures are exchanged, cooperative studies are done (the Arizona station currently with the Iowa and Clay Center stations) and papers are co-authored (two papers in press).

           2.      Regulation of the calpain system in platelets.

     With J.E.B. Fox, the Cleveland Clinic Foundation, Cleveland, OH; one paper published in 2000. 

           3.      Localization of the Calpains and Calpastatin in Human Dystrophic Muscle.

With Dr. Toshihide Kumamoto, the Third Department of Internal Medicine; Oita Medical University, Oita, Japan. We are sending monoclonal and polyclonal antibodies specific for the two calpain and calpastatin to Dr. Kumamoto, who is using these reagents to localize these proteins in muscle from normal human patients and human patients having different muscular dystrophies. Two papers have been published from this work. 

           4.   Role of the Calpains and Calpastatin in Skeletal Muscle Growth.

With Drs. A. Ouali and R. G. Taylor, INRA, Clermont-Ferrand, France. We are exchanging antibodies to the calpains and calpastatin with Drs. Ouali and Taylor, and I spent 2-1/2 months from 22 May - 3 August, 1998 in the INRA laboratory at Clermont-Ferrand. Two papers have been published. Consortium with INRA, USDA, Clay Center, Bordeaux and Tucson on Genetics in Meat Animal Species being planned; first meeting 11-12 April, Paris. 

           5.      Calpains on Ischemic and Reperfused Hearts

With Dr. Robert S. Decker, Departments of Medicine and Molecular Biology, Northwestern University Medical School. We are doing calpain localization and assays in the infarcted areas surrounding ischemic cardiac tissue.

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