Darrel E. Goll, Professor
Date of First UA Appointment: 1 July, 1976

Current Research Interests

Research has focused on the role of protein degradation in growth and muscle diseases, with emphasis on the calpain system, and on properties of cytoskeletal proteins involved in membrane/cytoskeleton interaction, with emphasis on Z-disks in striated muscle.

Research in Progress:

• Molecular Mechanisms Regulating Skeletal Muscle Growth and Differentiation
  
• Role of the Calpain System in Skeletal Muscle Development and Growth
  
• Role of the Calpain System in Muscle Degeneration
  
• Postmortem Changes in The Calpain System and Their Relation to Tenderness
  
• The Calpain System in Health and Disease 
  
  
  Peer-reviewed publications

  1.      Thompson, V.F., and Goll, D.E. (2000). Purification of µ-calpain, m-calpain and calpastatin from animal tissues. In “Methods in Molecular Biology”, Calpain Methods and Protocols (Elce, J.S., ed.), Humana Press, Inc., Totowa, New Jersey pp. 3-16. 

  2.      Thompson, V.F., Lawson, K., and Goll, D.E. (2000) Effect of µ-calpain on m-calpain. Biochem. Biophys. Res. Comm. 267, 495-499. 

  3.      Bialkowska, K., Kulkani,S., Du, X., Goll,D.E., Saido,T.C., and Fox, J.E.B. (2000) Evidence that $3 integrin-induced Rac activation involves the calpain-dependent formation of integrin clusters that are distinct from focal complexes and focal adhesions that form as Rac and RhoA become active. J. Biol. Chem. 151, 685-695. 

  4.    Thompson, V.F., Saldańa, S.,Cong, J., and Goll, D.E. (2000) A BODIPY fluorescent microplate assay for measuring activity of calpains and other proteases. Anal. Biochem. 279, 170-178.  

  5.   Delgado, E.F., Geesink,G.H., Marchello, J.A., Goll, D.E., and Koohmaraie,M. (2001) The calpain system in three muscles of normal and callipyge sheep. J. Anim. Sci. 79, 398-412. 

  6.      Delgado, E.F., Geesink, G.H., Marchello, J.A., Goll,D.E., and Koohmaraie, M. (2001) Properties of myofibril-bound calpain activity in longissimus muscle of callipyge and normal sheep. J. Anim. Sci. 79 (in press). 

  7.   Cottin, P., Thompson, V.F., Sathe, S.K., Szpacenko, A., and Goll, D.E. (2001) Autolysis of µ- and m-calpain from bovine skeletal muscle. Biol. Chem. (in press).
  

Other activities:

Our laboratory sends samples of purified m-calpain, m-calpain, and calpastatin and antibodies, both monoclonal and polyclonal to these three proteins to a number of laboratories in the U.S. and Europe (in addition to those laboratories with whom we have established collaborative efforts). We accommodate approximately 10-15 such requests each year. We have developed a formal agreement with Affinity Bioreagents, Inc. (ABR) in Golden, Colorado to commercially market six of our monoclonal antibodies to the calpains and calpastatin.

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