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ENTO446/EIS 546 Insect Pathogens:
Biocontrol Agents and Biological Models


Department of Entomology
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ENTO446/546 Home  •   Schedule  •  Course Dynamics  •  Lectures/Labs (handouts)

Spring 2010

Course Schedule

Lectures: Mondays (M) and Wednesdays (W) 1:00-2:00 PM, Forbes 412
Laboratory/Discussion: Tuesdays (T) 2:30-4:30 PM, Marley 212

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Date Day Lecture/ Lab/ Discussion Topic Reading
1/13 W Lecture 1 Introduction. Discussion of course syllabus and course dynamics.  
1/18 M Martin Luther King Day No classes  
1/19 T Lab 1 Examination and diagnosis of diseased insects-recognition of symptoms, and signs of infection. Insect sybionts.  
1/20 W Lecture 2 History of insect pathology. Characteristics of insect pathogens. General concepts on diagnosis of insect diseases. Association between insects
and non-pathogenic organisms (pdf)
1/25 M Lecture 3 Associations between insects and non pathogenic microorganisms  
1/26 T Lab 2 Isolation of entomopathogens from soil samples. Initial handling of diseased insects.  
1/27 W Lecture 4 Bacteria I. Classification. General characteristics. Historical aspects. Spore-forming bacteria. Symptoms and pathology. Host range.  
2/1 M Lecture 5 Bacteria II. Non-spore forming bacteria. Symptoms and pathology. Host range.  
2/2 T Lab 3/Disc. 1 Soil lab follow up. Bacteria diseases. Shapiro et al. 2005 (pdf)
2/3 W Lecture 6 Bacteria III. Toxins. Crystal proteins. Biotechnology and Bt. Insect Resistance. Vertebrate Pathogenicity.  
2/8 M Lecture 7 Protozoa I: Classification General characteristics. Historical aspects. Non-spore formers: Flagellates, Ciliates and Rhizopoda. Symptoms and pathology. Host range.  
2/9 T Lab 4/Disc. 2 Entomopathogenic bacteria Blanco et al. 2007 (pdf) , Heckel et al 2007 (pdf), Damgaard 2000 (pdf)
2/10 W Lecture 8 Protozoa II. Spore formers: Gregarines, Coccidia, Microsporidia. Symptoms and pathology. Host range.  
2/15 M Lecture 9 Protozoa III. Spore formers: Gregarines, Coccidia, Microsporidia. Molecular biology and Protozoa classification. Phylogenetics. Major protozoa considered in biological control and IPM.
2/16 T   Review of lectures 1-9
Lab 3 follow-up
 
2/17 W MIDTERM 1 To include all topics from lectures 1-9, and laboratories 1-4  
2/22 M Lecture 10 Fungi I. Classification. General characteristics. Historical aspects. Molecular biology and Fungi classification. Phylogenetics. Mastigomycetes. Symptoms and pathology. Host range.  
2/23 T Lab 5 Protozoa and Fungi I  
2/24 W Lecture 11 Fungi II. Zygomycetes, Ascomycetes, Symptoms and pathology. Host range.  
3/1 M Lecture 12 Fungi III. Basidiomycetes, Deuteromyces. Symptoms and pathology. Host range. Fungi considered in biological control and IPM .
3/2 T Lab 6 /Disc. 3 Fungi II Holder & Keyhani 2005 (pdf)
3/4 W Lecture 13 Nematodes I. Classification. General characteristics. Historical aspects. Nematode-insect associations. Non-pathogenic groups (phoretic, facultative and obligate parasites). Symptoms and pathology. Host range.  
3/8 M Lecture 14 Nematodes II. Insects as vectors of nematode parasites of vertebrates. Symptoms and pathology. Host range.  
3/9 T NO CLASS    
3/10 W Lecture 15 Nematodes III. Entomopathogenic nematodes: Steinernematidae, Heterorhabditidae. Symptoms and pathology. Host range.  
3/15 M SPRING BREAK No classes  
3/16 T SPRING BREAK No classes  
3/17 W SPRING BREAK No classes  
3/22 M Lecture 16 Viruses I. Historical aspects. Classification. Phylogenetics. Characteristics of NPV and GV. Symptoms and pathology. Host range.  
3/23 T Lab 7 Nematodes  
3/24 W Lecture 17 Viruses II. Other DNA viruses. RNA viruses. Molecular Biology of Baculoviruses. Recombinant Baculovirus Insecticides.  
3/29 M MIDTERM 2 To include all topics from lectures 10-16 and laboratories 5, 6  
3/30 T Disc. 4 Nematodes Lewis, et al. 2006, Arthurs & Heinz 2003
3/31 W Lecture 18 Viruses III. Virus considered in biological control and IPM. Examples of success in forestry and agricultural settings  
4/5 M Lecture 19 Insect defense mechanisms against pathogens. Host resistance.  
4/6 T Disc. 5 Insect defense mechanisms Vallet-Gely, et al. 2008, Narayanan 2004
4/7 W Lecture 20 Epizootiology. Key factors in epizootiology. The environment. Modeling. Examples  
4/12 M Lecture 21 Microbial Control I. Economic threshold. Factors affecting efficacy. Safety. Hajeck chapters 4 & 5
4/13 T Disc. 6 Plant-mediated effects on pathogens van Tol, et al. 2001, Cory, et al. 2006, QUESTIONS
4/14 W Lecture 22 Microbial Control II. Advantages and disadvantages of microbial control. Long and Short-term control. Insect Pathogens and Integrated Pest Management  
4/19 M Lecture 23 Entomopathogens and the genomics era.  
4/20 T Disc. 7 Insect pathogens and GMOs Thritle et al., 2003, Sears et al. 2001, Losey et al. 1999
4/21 W Lecture 24 Insect pathogens and GMOs. Strain improvement. Current examples and applications. Entomopathogens as biological models  
4/26 M   Student presentations (I)  
4/27 T   NO CLASS  
4/28 W   Student presentations (II)  
5/3 M Course review Review of topics learned - course evaluation  
5/5 W MIDTERM 3 To include all topics from lectures 17-25  
5/10 M FINAL EXAM To include all topics from lectures 1-25, and laboratories 1-6.  

 

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