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Oligonucleotide Microarray Workshop: March 2003 |
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ARABIDOPSIS MICROARRAY WORKSHOP
March 16-19, 2003
University of Arizona
Tucson, Arizona
March 16-19, 2003
University of Arizona
Tucson, Arizona
This three-day workshop, with 20 participants, comprised a combination of lectures and hand-on laboratory sessions, in which participants were introduced to use of microarrays in genomic research. The laboratory work primarily employed Arabidopsis 26,000 element 70-mer oligonucleotide microarrays (for details of these arrays, see www.ag.arizona.edu/microarray).
Topics included:
- Experimental design.
- Probe preparation and microarray printing.
- Target preparation.
- Microarray hybridization, using manual and automated methods.
- Array scanning.
- Data extraction and analysis.
Group photographs ( 1, 2, 3 )
Other digital pictures
Industrial participants:
| Company |
Contact |
Presentation |
| Agilent |
Dahlia Riley (Dahlia_riley@agilent.com) |
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| BioDiscovery |
Pankaj Prakash (pprakash@biodiscovery.com) |
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| GeneMachines/Genomic Solutions |
Jacques Fayet-Faber (jacques@genemachines.com) |
|
| Genicon |
Rachel Formosa (rformosa@geniconsciences.com) |
Powerpoint |
| Nanodrop |
Walter Schick (walterschick@ca.astound.net) |
|
| Qiagen-Operon |
Nisha Sahay (n.sahay@us.qiagen.com) Lewis Vann (l.vann@us.qiagen.com) |
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| Telechem |
Todd Martinsky (todd@arrayit.com) |
Powerpoint |
| Ventana |
Gabriel Longorio (glongoria@ventanamed.com) |
Summary:
Twenty participants attended the workshop, drawn from seven countries. Participants were divided into four groups, and each group prepared Cy3 and Cy5 RNA targets from Arabidopsis tissues. The degree of RNA and target purity and integrity was evaluated using the NanoDrop spectrophotometer, agarose gel electrophroesis, and the Agilent Bioanalyzer. Targets were hybridized to Arabidopsis oligonucleotide microarrays (print run 1.11.1). All groups obtained hybridization signals appropriate for the tissue from which the RNA was extracted.
For further workshop details, please contact David Galbraith (galbraith@arizona.edu).
David W. Galbraith
University of Arizona
Department of Plant Sciences
303 Forbes Building
Tucson Arizona 85721
Tel: (520) 621-9153; Fax (520) 621-7186
Email: galbraith@arizona.edu
Questions or comments
should be addressed to:David Galbraith
Page
last updated April 5, 2003