PLANT AND ANIMAL LONG-OLIGONUCLEOTIDE
MICROARRAY WORKSHOP

January 7-12, 2007

University of Arizona
Tucson, Arizona

This workshop comprised a combination of lectures and hand-on laboratory sessions.  The 40 participants employed Arabidopsis and maize (plant side) and human (animal side) whole genome 70-mer oligonucleotide microarrays in their laboratory work (for details of the plant arrays see: Arabidopsis, maize).  The workshop was aimed at the goal of obtaining optimal results using oligonucleotide-based microarrays. 

The workshop was divided into two parts:  Part I covered wet-lab aspects of microarray target production, microarray hybridization, and scanning.  It started with registration on Sunday, January 7, from 2-4PM, with the first formal activities at 5PM, and ended on Wednesday evening.  Part II covered data extraction, statistical analysis, and experimental design, and ran on Thursday and Friday.  The workshop ended at 5pm on Friday.

Note:  The number of airline connections from Tucson to San Diego on Friday evening allowed workshop participants convenient access to the Plant and Animal Genome XV Meeting (January 13-17, 2007).

Specific topics included:

• Experimental design.
• Probe preparation and microarray printing.
• Microarray rehydration and probe immobilization.
• Target preparation, including RNA extraction, direct and indirect labeling, and amplification techniques.
• Microarray hybridization.
• Array scanning and data extraction.
• Data analysis and archiving.

Workshop photographs

Workshop lectures

The next microarray workshop will be held from January 6-11, 2008. To register for the workshop and for further enquiries, please contact
David Galbraith (galbraith@arizona.edu).

For information about local accommodation, please follow this link. 

David W. Galbraith
University of Arizona
Department of Plant Sciences
303 Forbes Building
Tucson Arizona 85721
Tel: (520) 621-9153; Fax (520) 621-7186