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Dr.
Bentley A. Fane
Professor
Division of Plant Pathology and Microbiology
1657 N. Helen St.
Keating Building, Room 219
Tucson, Arizona 85721
Phone: (520) 626-6634
Fax: (520) 626-4824
Email: bfane@u.arizona.edu
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Visit
these web-sites to learn more about Dr. Fane and his research
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| Background
and Interests |
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Bentley
Fane received a Ph.D. in Biology from Massachussetts Institute of
Technology in 1988. His research interests include molecular mechanism
of virus morphogenesis.
The
proper assembly of proteins and nucleic acids into biologically
active virions involves numerous and diverse macromolecular interactions.
While structural proteins, those found in the mature virion, must
correctly interact, proper morphogenesis is equally dependent on
scaffolding proteins. Analogous to scaffoldings used in building
construction, these proteins ensure the integrity and efficiency
of viral morphogenesis but they are not found in the final product.
In addition to scaffolding proteins, the morphogenesis of single-stranded
icosahedral viruses may be influenced by the association of the
genome to the inner surface of the capsid. The broad objective of
our research program is to elucidate the molecular mechanisms involved
in scaffolding-mediated and genome-mediate morphogenesis within
the Microviridae system. In addition, we are also beginning to examine
the biology of viruses that infect obligate intracellular parasitic
bacteria.
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| Publications |
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1.
Cherwa, J. E., Jr., Uchiyama, A. & Fane, B. A.
(2008). Scaffolding proteins altered in the ability to perform a conformational
switch confer dominant lethal assembly defects. J Virol 82, 5774-80.
2. Salim, O., Skilton, R. J., Lambden, P. R., Fane, B. A.
& Clarke, I. N. (2008). Behind the chlamydial cloak: the replication
cycle of chlamydiaphage Chp2, revealed. Virology 377, 440-5.
3. Uchiyama, A., Chen, M. & Fane, B. A. (2007).
Characterization and function of putative substrate specificity domain
in microvirus external scaffolding proteins. J Virol 81, 8587-92.
4. Chen, M., Uchiyama, A. & Fane, B. A. (2007).
Eliminating the requirement of an essential gene product in an already
very small virus: scaffolding protein B-free oX174, B-free. J Mol
Biol 373, 308-14.
5. Skilton, R., Cutcliffe, L. T., Pickett, M. A., Lambden, P. R.,
Fane, B. A. & Clarke, I. N. (2007). Intracellular
parasitism of chlamydiae: the specific infectivity of chlamydiaphage
Chp2, in Chlamydophila abortus. J Bacteriol 189, 4957-9.
6. Fane, B. A., Brentlinger, K. L., Burch, A. D.,
Hafenstein, S. L., Moore, E., Novak, C. R. Uchiyama, A. (2006). øX174
et al. In The Bacteriophages Second edit. (Calendar, R., ed.), pp.
129-145. Oxford Press, London.
7. Uchiyama, A. & Fane, B. A. (2005). Identification
of an interacting coat-external scaffolding protein domain required
for both the initiation of phiX174 procapsid morphogenesis and the
completion of DNA packaging. J Virol 79, 6751-6.
8. Clarke, I. N., Cutcliffe, L. T., Everson, J. S., Garner, S. A.,
Lambden, P. R., Pead, P. J., Pickett, M. A., Brentlinger, K. L. &
Fane, B. A. (2004). Chlamydiaphage Chp2, a skeleton
in the phiX174 closet: scaffolding protein and procapsid identification.
J Bacteriol 186, 7571-4.
9. Morais, M. C., Fisher, M., Kanamaru, S., Przybyla, L., Burgner,
J., Fane, B. A. & Rossmann, M. G. (2004). Conformational
switching by the scaffolding protein D directs the assembly of bacteriophage
phiX174. Mol Cell 15, 991-7.
10. Hafenstein, S. L., Chen, M. & Fane, B. A.
(2004). Genetic and functional analyses of the oX174 DNA binding protein:
the effects of substitutions for amino acid residues that spatially
organize the two DNA binding domains. Virology 318, 204-13.
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