MARK R. RILEY

Assistant Professor

Department of Agricultural and Biosystems Engineering

Shantz Bldg. Room 403, University of Arizona; Tucson, AZ 85721-0038

(520) 626-9120 (Ph) or (520) 621-3963 (FAX) email: riley@ag.arizona.edu

Education

Ph. D. in Chemical and Biochemical Engineering, October, 1995; Rutgers University, Piscataway, NJ; Dissertation: “Diffusion, reaction, and cell proliferation in immobilized cell systems”

M. S. in Chemical and Biochemical Engineering, January, 1994; Rutgers University, Piscataway, NJ.

B. S. E. Cum Laude, in Chemical Engineering, August, 1990; University of Michigan, Ann Arbor, MI.

Academic experience

April 1997 - present, Assistant Professor, Department of Agricultural and Biosystems Engineering, University of Arizona.

August 1997 - present, Faculty member of the Graduate Interdisciplinary Program in Biomedical Engineering, University of Arizona. Chair of the Activities Subcommittee (Fall 1999 -present).

August 1995 - March 1997, Post-doctoral researcher, Department of Chemical and Biochemical Engineering and Department of Chemistry, University of Iowa, Supervisors - Dr. Mark Arnold and Dr. David Murhammer.

January 1992 - July 1995, Graduate dissertation research, Department of Chemical and Biochemical Engineering, Rutgers University. Advisors - Dr. Fernando Muzzio and Dr. Helen Buettner.

February 1989 - July 1990, Undergraduate research, Cellular Bioengineering Laboratory, Department of Chemical Engineering, University of Michigan. Advisor - Dr. Bernhard Palsson.

Industrial internships

Summer 1993, 1994, Exxon Research and Engineering, Annandale, NJ. Advisor - Dr. Sebastian Reyes.

Summer 1991, Ortho Pharmaceuticals, Raritan, NJ. Advisor - Dr. Robert Zivin.

Teaching experience

Fall 2000,2001, Agricultural and Biosystems Sophomore Seminar, ABE 296, 8-10 students, University of Arizona.

Spring 1999, 2001 Engineering of Biological Processes, ABE 485 / 585, 22 students, University of Arizona.

Summer 1998-2001, Biotechnology and Bioengineering, College of Agriculture, ABE 197B, University of Arizona.

Horizon's Unlimited program (9-15 students).

Spring 1998, 2000, 2002, Agricultural Bioengineering, Ag. and Biosystems Engineering, 487/587, University of Arizona.

Fall 1997-200, Introduction to Engineering, College of Engineering and Mines, University of Arizona.

Fall and Spring 1997-2001 Agri-Biosystems Engineering Design, 1-2 Projects for 2-7 students, University of Arizona.

Fall 1994, Instructor of undergraduate class in momentum transport phenomena, Department of Chemical and Biochemical Engineering, Rutgers University.

Spring 1993, Fall 1993, and Spring 1994, Department of Chemical and Biochemical Engineering, Rutgers University. Teaching assistant for graduate and undergraduate classes in transport phenomena.

Fall 1990, Department of Biochemistry, University of Michigan. Advisor - Dr. Robert Beyer. Undergraduate teaching assistant for a self-paced biochemistry class.

Refereed Publications (20 total)

Okeson, C.D., M. R. Riley, A. Fernandez, J.O.L. Wendt, “Impact of the composition of combustion generated fine particles on epithelial cell toxicity: influences of metals and metabolism,” accepted for publication in Chemosphere, July, 2002.

Canaves, L.C., M.R. Riley, “FT-NIR spectroscopic analysis of nitrogen in cotton leaves,” accepted for publication in Applied Spectroscopy, to appear in issue 56 (11), November, 2002.

Okeson, C.D. and M.R. Riley, "Glutamine replenishment and ammonia removal in hybridoma cell cultures via immobilized glutamine synthetase," Biochemical Engineering Journal, 9, 2, 125-133 (2001).

Riley, M.R., H.M Crider, M.E. Nite, R.A. Garcia, J. Woo, R. M. Wegge, "Simultaneous measurement of 19 components in animal cell culture media by near infrared spectroscopy," Biotechnology Progress, 17, 2, 376-378, (2001).

Frazier, B.L., P. Larmour, and M.R. Riley, "Noninvasive measurement of effective diffusivities in cell immobilization gels through use of near infrared spectroscopy," Biotechnology and Bioengineering, 72, 3, 364-368, (2001).

Riley, M.R., and H. M. Crider, “The effect of analyte concentration range on measurement errors obtained by NIR spectroscopy,” Talanta, 52, 473-484, (2000).

Riley, M.R., M.A. Arnold, and D.W. Murhammer, “Effect of sample complexity on quantification of analytes in aqueous samples by near infrared spectroscopy,” Applied Spectroscopy, 54, 2, 255-261, (2000).

Riley, M.R., C.D. Okeson, and B.L. Frazier, “Rapid calibration development for near infrared spectroscopic monitoring of mammalian cell cultivations,” Biotechnology Progress, 15, 6, 1133-1141, (1999).

Riley, M.R., F.J. Muzzio, and S.C. Reyes, “Experimental and modeling studies of diffusion in immobilized cell systems: a review of the recent literature and patents,” invited and peer reviewed article Applied Biochemistry and Biotechnology, 80, 2, 151-188, (1999).

Riley, M.R., M.A. Arnold, and D.W. Murhammer, “Matrix enhanced calibration procedure for multivariate calibration models with near-infrared spectra,” Applied Spectroscopy, 52, 10, 1339-1347 (1998).

Riley, M.R., M.A. Arnold, and D.W. Murhammer, E.L. Walls, and N. DelaCruz, “Adaptive calibration scheme for quantification of metabolites in insect cell media by near infrared spectroscopy,” Biotechnology Progress, 14, 527-533 (1998).

Riley, M.R., F.J. Muzzio, and S.C. Reyes, “Calculation of effective diffusivities and rates of reaction in immobilized cell systems using finite difference methods,” Computers and Chemical Engineering, 22: (4-5) 525-534 (1998).

Riley, M.R., M. Rhiel, X. Zhou, M.A. Arnold, and D.W. Murhammer, “Simultaneous monitoring of glucose and glutamine in insect cell cultures by NIR spectroscopy,” Biotechnology and Bioengineering, 55, 1, 11-15 (1997).

Riley, M.R., F.J. Muzzio, and S.C. Reyes, “Effect of oxygen limitations on monoclonal antibody production by immobilized hybridoma cells,” Biotechnology Progress, 13, 3, 301-310 (1997).

Riley, M.R., F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “A simple correlation for predicting effective diffusivities in immobilized cell systems,” Biotechnology and Bioengineering, 49, 223-227 (1996).

Riley, M.R., F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “The effect of structure on diffusion and reaction in immobilized cell systems,” Chemical Engineering Science, 50, 21, 3357-3367 (1995).

Riley, M.R., H.M. Buettner, F.J. Muzzio, and S.C. Reyes, “Monte Carlo simulation of diffusion and reaction in two-dimensional cell structures,” Biophysical Journal, 68, 5, 1716-1726 (1995).

Riley, M.R., F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Diffusion in heterogeneous media: application to immobilized cell systems,” AIChE J, 41, 3, 691-700 (1995).

Riley, M.R., F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Monte Carlo calculation of effective diffusivities in two- and three-dimensional heterogeneous materials of variable structure,” Physical Review E, 49, 4, 3500-3503 (1994).

Ozturk, S.S., M.R. Riley, and B.Ǿ. Palsson, “Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production,” Biotechnology and Bioengineering, 39, 418-431 (1992).

Boonmung, S., M.R. Riley, “Quantitative Analysis of Added Ammonium and Nitrate in Silica Sand Using Diffuse Reflectance Infrared Spectroscopy,” submitted to Applied Spectroscopy, July 2002.

Riley, M.R., D.E. Boesewetter, A.M. Kim, F.P. Sirvent, “Synergistic effects of metals on rat lung epithelial cells,” to be submitted to Toxicology, September, 2002.

Articles in books (not peer reviewed)

M.R. Riley, "Monitoring of animal cell cultivations by near-infrared spectroscopy," Recent Research Developments in Biotechnology & Bioengineering, Research Signpost Pub., Trivandrum, India, 3, 143-166 (2000).

M.R. Riley, "Enzyme Kinetics," Invited chapter to appear in Encyclopedia of Agricultural and Food Engineering Marcel Dekker Pub., New York, NY. To appear in Dec, 2001, 5 pages.

M.R. Riley, “Instrumentation and Process Control,” chapter in Cell Culture Technology Marcel Dekker Pub., New York, NY. To appear in Jan 2002, 54 pages.

Presentations at Technical Meetings (45 total)

Garcia, R.A [Speaker],. and M.R. Riley, “Enhancing the activity of lipase enzymes for use in non-aqueous solutions,” presented at the 2001 ASAE Meeting, Monday, July 30.

Boonmung, S. [Speaker], and M.R. Riley, “NIR spectroscopy to quantify nitrogen content of soils,” presented at the 2001 ASAE Meeting.

Canaves, L.C. [Speaker], and M.R. Riley, “Cotton nitrogen stress determination by NIR spectroscopy,” to be presented at the 2001 ASAE Meeting.

Okeson, C.D. [Speaker], M.R. Riley, Art Fernandez, Jost O. L. Wendt, and Mark L. Witten, “Impact of the composition of combustion generated fine particles on epithelial cell toxicity: influence of metals on metabolism,” NIEHS Conference, North Carolina, May, 2001.

M.R. Riley [Speaker] and C.D. Okeson, “Toxicity monitoring using lung cell cultures,” to be presented at the 2001 IBE Meeting.

Wendt, Jost O.L. [Speaker], Art Fernandez, Mark L. Witten, Sheng Wang, M. R. Riley, Carl Okeson, William P Linak, C. Andrew Miller, “On the Generation and Subsequent Health Effects of Fuel Combustion Generated Particulates: The Roles of Zinc and Vanadium,” Presented at the 13^th International Flame Research Foundation Members’ Conference, Noordwijkerhout, The Netherlands, May 15-18, 2001.

M R. Riley, [Speaker], Heather M. Crider, Megan E. Nite, Rafael A. Garcia, Rachel M. Wegge, "Near-Infrared Spectroscopy for monitoring animal cell cultivations," presented at the 2000 AIChE Conference.

M. R. Riley [Speaker], Loreto Canaves, and Rafael Garcia, "Application of Fourier-transform near-infrared spectroscopy to perform noninvasive measurements," poster presented at the 2000 ASAE meeting.

Carl D. Okeson [Speaker] and M R. Riley, "Ammonia removal and glutamine replenishment in hybridoma cell culture media via conversion by encapsulated glutamine synthetase," presented at the University of Arizona Biomedical Engineering Program Poster Session, 3/3/2000.

M R. Riley [Speaker], H.M. Crider, M.E. Nite, R.A. Garcia, J. Woo, and R.M. Wegge, "Noninvasive monitoring of nutritional requirements of hybridoma cells by NIR spectroscopy," poster presented at the ACS 2000 National meeting, San Francisco, CA.

Carl D. Okeson [Speaker] and M R. Riley, "Glutamine synthetase for the replenishment of hybridoma cell culture media," poster presented at the ACS 2000 National meeting, San Francisco, CA.

M. R. Riley, Heather M. Crider, and Megan Nite, "Simultaneous Measurement of 19 Components in Animal Cell Culture Media by Near Infrared Spectroscopy," presented at the 1999 AIChE Meeting, Dallas, TX.

Brenna L. Frazier, Paul A. Larmour, and M.R. Riley [Speaker], "Noninvasive determination of effective diffusivities in immobilized cell systems," presented at the 1999 AIChE Meeting, Dallas, TX.

Carl D. Okeson, M.R. Riley [Speaker], "Conversion of ammonia to glutamine in hybridoma culture media via immobilized glutamine synthetase," poster presented at the 1999 AIChE Meeting, Dallas, TX.

Riley, M.R., “A Kinetic Model of Diffusion and Reaction in Immobilized Cells Systems," presented at the ASAE National Meeting, Toronto, Canada (1999).

Riley, M.R., “Automated calibration development for noninvasive monitoring of chemical concentrations by near infrared spectroscopy,” Presented at the 1^st Annual AZ Biotechnology Symposium, Tempe, AZ (1999).

Riley, M.R., [Speaker], Carl D. Okeson, and Brenna L. Frazier, “Development of Calibration Models For Spectroscopic Monitoring of Hybridoma Cell Cultivations,” presented at the ACS Meeting (1998).

Riley, M.R., [Speaker], Carl D. Okeson, and Brenna L. Frazier, “Noninvasive quantification of cell culture components by near infrared spectroscopy,” presented at the AIChE National Meeting (1998).

Riley, M.R., [Speaker], Heather M. Crider, “A Metabolic Model for Immobilized Hybridoma Cells,” poster presented at the AIChE National Meeting (1998).

Riley, M.R., "Integration of experiential learning in an agricultural bioengineering course," poster presented at the Western Regional Teaching Symposium, Idaho (1998).

Riley, M.R., “New methods for calibrating infrared spectroscopic measurements for bioreactor monitoring,” presented at a joint session of IBE-ASAE in Orlando, FL (1998).

Riley, M.R. [Speaker], D.W. Murhammer, M.A. Arnold, E. Walls, and N. DelaCruz, “An adaptive calibration scheme for NIR measurements of metabolites in insect cell bioreactors,” presented at the AIChE National Meeting, Los Angeles, CA (1997).

Rhiel, M. [Speaker], M.R. Riley, H. Chuang, S. Spear, P. Palechek, M.B. Cohen, M.A. Arnold, and D.W. Murhammer, “Continuous, noninvasive monitoring of bioreactors,” presented at ACHEMA 97, Frankfurt, Germany (1997).

Riley, M.R., “Near Infrared Spectroscopy for Noninvasive Bioreactor Monitoring ,” presented at the Institute for Biological Engineering National Meeting, Minneapolis, MN, (1997).

Riley, M.R., “Nutrient transport in immobilized cell systems,” presented at the FASEB Experimental Biology National Meeting, New Orleans, LA, (1997).

Rhiel, M, Riley, M.R., Arnold, M.A., Murhammer, D.W., “Evaluation of near-infrared spectroscopy for bioreactor monitoring” presented at the American Chemical Society Annual Meeting, San Francisco, CA, (1997).

Murhammer, D.W. [Speaker], M.R. Riley, M. Rhiel, M.A. Arnold, E. Walls, and N. DelaCruz, “Simultaneous monitoring of multiple cellular metabolites in bioreactors by NIR spectroscopy,” presented at the American Chemical Society Annual Meeting, San Francisco, CA, (1997).

Riley, M.R. [Speaker], M. Rhiel, M.A. Arnold, and D.W. Murhammer, “Monitoring metabolite concentrations in insect and mammalian cell bioreactors by NIR spectroscopy,” poster presented at the AIChE National Meeting, Chicago, IL, (1996).

Rhiel, M. [Speaker], M.R. Riley, X. Zhou, M.A. Arnold, and D.W. Murhammer, “Sensitivity and selectivity of near-infrared spectroscopy methods for process monitoring,” presented at the AIChE National Meeting, Chicago, IL, (1996).

Odde, D.J. [Speaker] and M.R. Riley, “Tubulin transport in microtubule assembly,” poster presented at the AIChE National Meeting, Chicago, IL, (1996).

Riley, M.R. [Speaker], M.A. Arnold, and D.W. Murhammer, “Non-invasive measurement of glucose concentrations by near infrared spectroscopy,” poster presented at the Biomedical Engineering Society Fall Meeting, State College, PA, (1996).

Odde, D.J. [Speaker] and M.R. Riley, “The role of diffusion in microtubule assembly,” presented at the Biomedical Engineering Society Annual Fall Meeting, State College, PA, (1996).

Riley, M.R. [Speaker] and M.A. Arnold, “Analysis of near infrared spectroscopy for industrial monitoring of aqueous processes,” presented at the Center for Process Analytical Chemistry Meeting, Seattle, WA (1996).

Riley, M.R. [Speaker], M. Rhiel, X. Zhou, M.A. Arnold, and D.W. Murhammer, “NIR spectroscopy for monitoring metabolite concentrations in insect cell bioreactors,” poster presented at the Biocatalysis and Bioprocess Conference, Iowa City, IA, (1996).

Riley, M.R. [Speaker], M. Rhiel, X. Zhou, M.A. Arnold, and D.W. Murhammer, “Simultaneous monitoring of glucose and glutamine in insect cell cultures by NIR spectroscopy,” presented at the Iowa Academy of Sciences Annual Meeting, Indianola, IA (1996).

Rhiel, M. [Speaker], M.R. Riley, X. Zhou, M.A. Arnold, and D.W. Murhammer, “Cell culture process monitoring by NIR spectroscopy,” poster presented at the Pittsburgh Conference, Chicago, IL (1996).

Riley, M.R. [Speaker], F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Nutrient diffusion and consumption in immobilized cell systems,” presented at the American Association of Pharmaceutical Scientists (AAPS) Eastern Regional Meeting, New Brunswick, NJ (1995).

Riley, M.R. [Speaker], H.M. Buettner, F.J. Muzzio, and S.C. Reyes, “Rate-controlling processes of reaction and diffusion in biological systems,” presented at the AIChE National Meeting, San Francisco, CA (1994).

Riley, M.R. [Speaker], F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Diffusion and reaction in heterogeneous biological materials,” presented at the AIChE National Meeting, San Francisco, CA (1994).

Riley, M.R. [Speaker], H.M. Buettner, F.J. Muzzio, and S.C. Reyes, “Diffusion and reaction of oxygen in biological systems,” presented at the Biomedical Engineering Society Annual Fall Meeting, Tempe, AZ (1994).

Riley, M.R. [Speaker], F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Diffusion and reaction in biological systems,” Biotechnology Minisymposium poster, Rutgers University, Piscataway, NJ (1994).

Riley, M.R. [Speaker], F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Analysis of diffusion, reaction, and cell proliferation in immobilized cell systems,” AIChE National Meeting, St. Louis, MO (1993).

Riley, M.R. [Speaker], F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Monte Carlo calculation of effective diffusivities of multiphase conductive media,” AIChE National Meeting, St. Louis, MO (1993).

Buettner, H.M. [Speaker], M.R. Riley, F.J. Muzzio, and S.C. Reyes, “Effective diffusivities of oxygen in immobilized cell systems: a Monte Carlo study,” Biomedical Engineering Society Annual Fall Meeting, Memphis, TN (1993).

Riley, M.R. [Speaker], M. Lovallo, F.J. Muzzio, H.M. Buettner, and S.C. Reyes, “Computational and experimental techniques for stationary-phase cell systems,” AIChE National Meeting, Miami Beach, FL (1992).

Sessions chaired at National Meetings

“Industrial Perspectives of Bioprocess Engineering,” at the 1998 IBE meeting, Orlando, FL.

“Advances in Bioprocess Engineering,” at the 1998 IBE meeting, Orlando, FL.

“Biomechanics and Biorheology,” co-chair with V.A. Barocas at the 1998 AIChE meeting in Miami, FL.

"Novel experimental methods in bioengineering," at the 1999 AIChE meeting in Dallas, TX.

"Advances in Biocatalysis & Protein Engineering," co-chair at the 1999 AIChE meeting in Dallas, TX.

"Advances in Bioprocessing, Sensors, Control & Optimization," co-chair at the 2000 AIChE meeting in Los Angeles, CA.

"Modeling and simulation", chair at the joint IBE-BMES meeting in Seattle, WA, Fall 2000.

"In Vitro and in Vivo Protein Folding and Aggregation" at the ACS meeting in San Diego (April 1-5, 2001) chair.

International Seminars

"Use of NIR spectroscopy in agricultural and biological engineering," February 1999, Tokyo University, Japan.

"Noninvasive measurement schemes for biological engineering," March 1999, Nagasaki University, Japan.

Invited Seminars

Abbott Laboratories, North Chicago, IL, February, 1995.

Merck and Co., Rahway, NJ, March, 1995.

Massachusetts Institute of Technology, Dept. of Chemical Engineering, June, 1995.

University of Florida, Department of Chemical Engineering, March, 1996.

University of Maryland, Chemical Engineering and Center for Agricultural

Biotechnology, April, 1996.

Purdue University, Dept. of Agricultural and Biological Engineering, May , 1996.

University of Louisville, Department of Chemical Engineering, June, 1996.

Drexel University, Department of Chemical Engineering, August 1996.

University of Arizona, Department of Agricultural and Biosystems Engineering,

September, 1996.

University of Kentucky, Biosystems and Agricultural Engineering, May, 2000.

Honors

Sigma Xi, The Scientific Research Society (8/2000 – present).

Alpha Epsilon, Agricultural Engineering Honor Society (1998-present).

Merck Excellence Fellowship (1993-1995).

NIH Biotechnology Pre-doctoral Fellowship (1990-1993).

B. S. E. Cum Laude, University of Michigan (1990).

Dean’s List - University of Michigan (1988, 1989).

Phi Lambda Upsilon - University of Michigan Chemical Honor Fraternity (1990).

Professional Associations

AIChE (American Institute of Chemical Engineers; 1992 - present).

BMES (Biomedical Engineering Society; 1994 - present).

ACS (American Chemical Society; 1996 - present).

Membership chair for ACS Biochemical Technology (BIOT) Division (5/98-present).

ASAE (American Society of Agricultural Engineering, 1997 - present)

Chair of the Biological Engineering Division (7/99 - 6/2000).

IBE (Institute for Biological Engineering, 1997- present)

IBE Councilor (2000 - 2002).

Scholarly activities

Editor for Patents and Literature Reviews for Applied Biochemistry and Biotechnology (Dec. 1998-present).