Properties of the Calpains

Home m-Calpain Domain Structure -Calpain Domain Structure Calpastatin Domain Structure

EC 3.4.22.17

Property Autolyzed -calpain -Calpain Autolyzed m-Calpain m-Calpain
Molecular Mass1 99,292 110,188 98,530 108,304
Molecular Mass, large subunit1 78,914 81,890 78,152 80,006
Molecular Mass, small subunit1 20,396 28,316 20,396 28,316
pH optimum2 7.6 7.5 7.4 7.6
[Ca2+] for half-maximal activity3  0.6-22.0 1-100 5-180 91-2500
[Ca2+] for binding Calpastatin4  0.042M 42M 24M 400M
Theoretical pI1 5.31 5.36 4.90 4.94
         

1Calculated using the Compute pI/Mw tool on the Expasy web site (http://ca.expasy.org/tools/pi_tool.html) and the human calpain sequences.

2Edmunds, T, Nagainis, PA, Sathe, SK, Thompson, VF, and Goll, DE (1991) Biochimica et Biophysica Acta, 1077, 197-208.

3Goll, DE, Kleese, WC, Kumamota, T, Cong, J, and Szpacenko, A (1989) In "Intracellular Proteolysis: Mechanisms and Regulation" (Katunuma, N and Kominami, E, eds.) Japan Scientific societies Press, Tokyo, Japan. pp. 80-91.

4Kapprell, H-P and Goll, DE (1989) J. Biol. Chem., 264, 17888-17896.

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This page was last updated on 25 September 2001 .

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