Deconvolving the microarrays:
The print format comprises a single array printed using 48 pins. The array contains 12 metarows and 4 metacolumns, with the subarrays containing 25 columns and 26 rows with a spacing of 170 x 165 microns. The dimensions of typical microarrays are given here (Powerpoint file). Red Reflectance and Syto-61 Stained and/or hybridization images are provided below.
The deconvolution files linking array elements with corresponding gene identifiers are provided below as Excel files, GenePix Array List files (GAL), and/or text files. Please check the numbers printed on the slide labels, and download the decode file that corresponds to those numbers.
For a discussion of QC aspects of deconvolution, please follow this link.
On all microarrays, we include array elements comprising the Stratagene SpotReportTM Oligo Array Validation System. This permits use of external normalization methods. The element locations are listed in the deconvolution files.
Complete array element sequence information is available from Operon at the following link: http://omad.operon.com/download/index.php
*TXT1, TXT2, GAL1 and GAL2 files provide Operon ID numbers
and At locus numbers respectively (note: these files were updated on
September 23, 2005).
3Used for QC purposes.
Questions, comments, or suspected
problems should be communicated to: